AR (phospho Ser94) rabbit pAb from ELK Biotechnology Read more ›
AR (phospho Ser94) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Androgen Receptor in IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoform
| Specificity | Phospho-AR (S94) Polyclonal Antibody detects endogenous levels of AR protein only when phosphorylated at S94. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HeLa cells, using Androgen Receptor (Phospho-Ser94) Antibody. The picture on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5937 |
| Target | Androgen Receptor |
| Research areas | >>Oocyte meiosis, >>Pathways in cancer, >>Chemical carcinogenesis - receptor activation, >>Prostate cancer |
| Reactivity | Human,Mouse |
| Applications | IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Androgen Receptor around the phosphorylation site of Ser94. AA range:66-115 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform 2: Mainly expressed in heart and skeletal muscle. ; Isoform 3: Expressed in basal and stromal cells of the prostate (at protein level). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
