IRF-3 rabbit pAb from ELK Biotechnology Read more ›
IRF-3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying IRF-3 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse, Pig. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],
| Specificity | IRF-3 Polyclonal Antibody detects endogenous levels of IRF-3 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HeLa cells, using IRF3 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using IRF3 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from K562 cells, using IRF3 Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5924 |
| Target | IRF-3 |
| Research areas | >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>Cytosolic DNA-sensing pathway, >>Alcoholic liver disease, >>Shigellosis, >>Pertussis, >>Yersinia infection, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Human cytomegalovirus infection, >>Influenza A, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 infection, >>Epstein-Barr virus infection, >>Human immunodeficiency virus 1 infection, >>Coronavirus disease - COVID-19, >>Viral carcinogenesis, >>Lipid and atherosclerosis |
| Reactivity | Human,Rat,Mouse,Pig |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IRF3. AA range:351-400 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed constitutively in a variety of tissues. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse | |||||||
| Pig |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Pig | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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