Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98874

ICAM-2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ICAM-2 rabbit pAb from ELK Biotechnology Read more ›

ICAM-2 rabbit pAb
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ICAM-2 rabbit pAb

Product Description

ICAM-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ICAM-2 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity ICAM-2 Polyclonal Antibody detects endogenous levels of ICAM-2 protein.
Validation evidence captions Western Blot analysis of 3T3 cells using ICAM-2 Polyclonal Antibody | Western blot analysis of the lysates from HT-29 cells using ICAM2 antibody.

Key Facts

Product typePrimary Antibody Target: ICAM-2
Collaborator ELK Biotechnology
Partner model ES5808
Target ICAM-2
Research areas>>Cell adhesion molecules, >>Natural killer cell mediated cytotoxicity
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ICAM2. AA range:151-200
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Aorta endothelial cell,Blood,Brain,Endothelial cell,Plasma,Spleen,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ICAM-2
Also known as
ICAM2; Intercellular adhesion molecule 2; ICAM-2; CD antigen CD102
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ICAM2. AA range:151-200
Observed band
30kD
Cellular localization
Membrane ; Single-pass type I membrane protein . Cell projection, microvillus . Co-localizes with RDX, EZR and MSN in microvilli. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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