BLNK (phospho Tyr96) rabbit pAb from ELK Biotechnology Read more ›
BLNK (phospho Tyr96) rabbit pAb is a research-use antibody from ELK Biotechnology for studying BLNK in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012],
| Specificity | Phospho-BLNK (Y96) Polyclonal Antibody detects endogenous levels of BLNK protein only when phosphorylated at Y96. |
|---|---|
| Validation evidence captions | Western Blot analysis of COLO205 cells using Phospho-BLNK (Y96) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BLNK (Phospho-Tyr96) Antibody | Immunohistochemistry analysis of paraffin-embedded human lymph node, using BLNK (Phospho-Tyr96) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from COLO205 cells, using BLNK (Phospho-Tyr96) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5692 |
| Target | BLNK |
| Research areas | >>NF-kappa B signaling pathway, >>Osteoclast differentiation, >>B cell receptor signaling pathway, >>Epstein-Barr virus infection, >>Primary immunodeficiency |
| Reactivity | Human,Mouse,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BLNK around the phosphorylation site of Tyr96. AA range:62-111 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Monkey | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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