GRB2 rabbit pAb from ELK Biotechnology Read more ›
GRB2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GRB2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
| Specificity | GRB2 Polyclonal Antibody detects endogenous levels of GRB2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of Hela cells using GRB2 Polyclonal Antibody diluted at 1:2000 | Western blot analysis of lysates from Jurkat cells, using GRB2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using GRB2 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5630 |
| Target | GRB2 |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>MAPK signaling pathway, >>ErbB signaling pathway, >>Ras signaling pathway, >>Chemokine signaling pathway, >>FoxO signaling pathway, >>Phospholipase D signaling pathway, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>Osteoclast differentiation, >>Focal adhesion, >>Gap junction, >>Signaling pathways regulating pluripotency of stem cells, >>JAK-STAT signaling pathway, >>Natural killer cell mediated cytotoxicity, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>Thermogenesis, >>Neurotrophin signaling pathway, >>Insulin signaling pathway, >>GnRH signaling pathway, >>Estrogen signaling pathway, >>Prolactin signaling pathway, >>Relaxin signaling pathway, >>Growth hormone synthesis, secretion and action, >>Alcoholism, >>Hepatitis C, >>Hepatitis B, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Pathways in cancer, >>Viral carcinogenesis, >>Proteoglycans in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GRB2. AA range:141-190 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Cajal-Retzius cell,Epidermis,Epithelium,Fetal brain cortex,Fe |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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