GPR35 rabbit pAb from ELK Biotechnology Read more ›
GPR35 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GPR35 in WB, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
function:Orphan receptor.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Expressed in all adult and fetal tissues examined, including pancreatic islets and skeletal muscle, with relatively higher levels in adult lung, small intestine, colon and stomach.,
| Specificity | GPR35 Polyclonal Antibody detects endogenous levels of GPR35 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using GPR35 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from LOVO cells, using GPR35 Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5614 |
| Target | GPR35 |
| Research areas | >>Neuroactive ligand-receptor interaction |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GPR35. AA range:51-100 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Predominantly expressed in immune and gastrointestinal tissues. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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