Pdcd-4 (phospho Ser457) rabbit pAb from ELK Biotechnology Read more ›
Pdcd-4 (phospho Ser457) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PDCD4 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010],
| Specificity | Phospho-Pdcd-4 (S457) Polyclonal Antibody detects endogenous levels of Pdcd-4 protein only when phosphorylated at S457. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293 cells using Phospho-Pdcd-4 (S457) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PDCD4 (Phospho-Ser457) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using PDCD4 (Phospho-Ser457) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from 293 cells treated with serum 20% 15', using PDCD4 (Phospho-Ser457) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5550 |
| Target | PDCD4 |
| Research areas | >>Proteoglycans in cancer, >>MicroRNAs in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PDCD4 around the phosphorylation site of Ser457. AA range:420-469 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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