c-Abl (phospho Tyr245) rabbit pAb from ELK Biotechnology Read more ›
c-Abl (phospho Tyr245) rabbit pAb is a research-use antibody from ELK Biotechnology for studying ABL1 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [pr
| Specificity | Phospho-c-Abl (Y245) Polyclonal Antibody detects endogenous levels of c-Abl protein only when phosphorylated at Y245. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HeLa cells, using c-Abl (Phospho-Tyr245) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from K562 cells treated with Insulin 0.01U/ml 15, using c-Abl (Phospho-Tyr245) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5403 |
| Target | ABL1 |
| Research areas | >>ErbB signaling pathway, >>Ras signaling pathway, >>Cell cycle, >>Axon guidance, >>Neurotrophin signaling pathway, >>Pathogenic Escherichia coli infection, >>Pathways in cancer, >>MicroRNAs in cancer, >>Chemical carcinogenesis - reactive oxygen species, >>Chronic myeloid leukemia, >>Viral myocarditis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human c-Abl around the phosphorylation site of Tyr245. AA range:196-245 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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