Seprase rabbit pAb

Seprase rabbit pAb

100%
Manufactured by:ELK Biotechnology
Save 20%RUO

Seprase rabbit pAb

Seprase rabbit pAb from ELK Biotechnology

SKU:
ABM108479
Manufacturer SKU
ES5204
Product type
Primary Antibodies
TargetSeprase
ApplicationsWB, IHC, IF/ICC, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed in adipose tissue. Expressed in the dermal fibroblasts in the fetal skin. Expressed in the granulation tissue of healing wounds and on reactive stromal fibroblast in epithelial cancers. Expressed in activated fibroblast-like synoviocytes from inflamed synovial tissues. Expressed in activated hepatic stellate cells (HSC) and myofibroblasts from cirrhotic liver, but not detected in normal liver. Expressed in glioma cells (at protein level). Expressed in glioblastomas and glioma cells. Isoform 1 and isoform 2 are expressed in melanoma, carcinoma and fibroblast cell lines.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Seprase rabbit pAb is a research-use antibody from ELK Biotechnology for studying Seprase in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014],

Additional antibody information

Specificity Seprase Polyclonal Antibody detects endogenous levels of Seprase protein.
Validation evidence captions Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. | Western Blot analysis of RAT-MUSCLE 293T cells using Seprase Polyclonal Antibody diluted at 1:2000 | Western blot analysis of 3T3 mouse-kidney 293T lysis using Seprase antibody. Antibody was diluted at 1:2000 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FAP-1 Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
Antibody Research
Also known as
FAP; Seprase; 170 kDa melanoma membrane-bound gelatinase; Fibroblast activation protein alpha; Integral membrane serine protease
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human FAP-1. AA range:331-380
Observed band
90kD
Cellular localization
[Prolyl endopeptidase FAP]: Cell surface . Cell membrane ; Single-pass type II membrane protein . Cell projection, lamellipodium membrane ; Single-pass type II membrane protein . Cell projection, invadopodium membrane ; Single-pass type II membrane protein . Cell projection, ruffle membrane ; Single-pass type II membrane protein . Membrane ; Single-pass type II membrane protein . Localized on cell surface with lamellipodia and invadopodia membranes and on shed vesicles. Colocalized with DPP4 at invadopodia and lamellipodia membranes of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Anchored and enriched preferentially by integrin alpha-3/bet
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 331-380
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Manufacturer SKU
ES5204
UniProt entry
Q12884
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00£176.00