EphB1/2/3 rabbit pAb from ELK Biotechnology Read more ›
EphB1/2/3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying EphB1/2/3 in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008],
| Specificity | EphB1/2/3 Polyclonal Antibody detects endogenous levels of EphB1/2/3 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of NIH/3T3 cells, using EPHB1/2/3 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EPHB1/2/3 Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5133 |
| Target | EphB1/2/3 |
| Research areas | >>Axon guidance |
| Reactivity | Human,Mouse |
| Applications | IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EPHB1/2/3. AA range:631-680 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Preferentially expressed in brain. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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