ATF-2 rabbit pAb from ELK Biotechnology Read more ›
ATF-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ATF-2 in IHC, IF, ELISA workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
activating transcription factor 2(ATF2) Homo sapiens This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014
| Specificity | ATF-2 Polyclonal Antibody detects endogenous levels of ATF-2 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HeLa cells, using ATF2 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES4806 |
| Target | ATF-2 |
| Research areas | >>MAPK signaling pathway, >>cGMP-PKG signaling pathway, >>PI3K-Akt signaling pathway, >>Longevity regulating pathway, >>Adrenergic signaling in cardiomyocytes, >>TNF signaling pathway, >>Thermogenesis, >>Dopaminergic synapse, >>Insulin secretion, >>Estrogen signaling pathway, >>Thyroid hormone synthesis, >>Glucagon signaling pathway, >>Aldosterone synthesis and secretion, >>Relaxin signaling pathway, >>Cortisol synthesis and secretion, >>Parathyroid hormone synthesis, secretion and action, >>Cushing syndrome, >>Growth hormone synthesis, >>Prion disease, >>Cocaine addiction, >>Amphetamine addiction, >>Alcoholism, >>Hepatitis B, >>Human cytomegalovirus infection, >>Human T-cell leukemia virus 1 infection, >>Viral carcinogenesis, >>Chemical carcinogenesis - receptor activation |
| Reactivity | Human,Rat |
| Applications | IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ATF2. AA range:456-505 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitously expressed, with more abundant expression in the brain. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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