CREB-1 (phospho Thr100) rabbit pAb from ELK Biotechnology Read more ›
CREB-1 (phospho Thr100) rabbit pAb is a research-use antibody from ELK Biotechnology for studying CREB-1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016],
| Specificity | Phospho-CREB-1 (T100) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at T100. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CREB (Phospho-Thr100) Antibody | Western blot analysis of lysates from HUVEC cells treated with etoposide 25uM 24H, using CREB (Phospho-Thr100) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4798 |
| Target | CREB-1 |
| Research areas | >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Adrenergic signaling in cardiomyocytes, >>Osteoclast differentiation, >>Antigen processing and presentation, >>TNF signaling pathway, >>Circadian rhythm, >>Circadian entrainment, >>Thermogenesis, >>Cholinergic synapse, >>Dopaminergic synapse, >>Insulin secretion, >>Estrogen signaling pathway, >>Melanogenesis, >>Thyroid hormone synthesis, >>Glucagon signaling pathway, >>Renin secretion, >>Aldosterone synthesis and secretion, >>Relaxin signaling pathway, >>Cortisol synthesis and secretion, >>Parathyroid hormone synthesis, secretion and action, >>Insulin resistance, >>Cushing syndrome, >>Growth hormone synthesis, >>Vasopressin-regulated water reabsorption, >>Huntington disease, >>Prion disease, >>Cocaine addiction, >>Amphetamine addiction, >>Alcoholism, >>Tuberculosis, >>Hepatitis B, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Human |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Thr100. AA range:71-120 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Eye,Placenta,Spleen,Testis, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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