Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98323

TCP-1 θ rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TCP-1 θ rabbit pAb from ELK Biotechnology Read more ›

TCP-1 θ rabbit pAb
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TCP-1 θ rabbit pAb

Product Description

TCP-1 θ rabbit pAb is a research-use antibody from ELK Biotechnology for studying TCP-1 θ in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1. [provided by RefSeq, Sep 2013],

Additional antibody information

Specificity TCP-1 θ Polyclonal Antibody detects endogenous levels of TCP-1 θ protein.
Validation evidence captions Western Blot analysis of HeLa cells using TCP-1 θ Polyclonal Antibody | Western blot analysis of CCT8 Antibody. The lane on the right is blocked with the CCT8 peptide. | Western blot analysis of the lysates from HeLa cells using CCT8 antibody.

Key Facts

Product typePrimary Antibody Target: TCP-1 θ
Collaborator ELK Biotechnology
Partner model ES4536
Target TCP-1 θ
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human CCT8. AA range:361-410
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Adipose tissue,B-cell lymphoma,Bone marrow,Chondrosarcoma,Epitheliu
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
TCP-1 θ
Also known as
CCT8; C21orf112; CCTQ; KIAA0002; T-complex protein 1 subunit theta; TCP-1-theta; CCT-theta; Renal carcinoma antigen NY-REN-15
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CCT8. AA range:361-410
Observed band
60kD
Cellular localization
Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, cilium basal body .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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