C/EBP α (phospho Thr226) rabbit pAb
C/EBP α (phospho Thr226) rabbit pAb from ELK Biotechnology Read more ›
Product Description
C/EBP α (phospho Thr226) rabbit pAb is an ELK Biotechnology antibody for research workflows involving C/EBP α.
Background
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013],
Additional antibody information
| Specificity | Phospho-C/EBP α (T226) Polyclonal Antibody detects endogenous levels of C/EBP α protein only when phosphorylated at T226. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using C/EBP-alpha (Phospho-Thr226) Antibody | Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using C/EBP-alpha (Phospho-Thr226) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
| Target | C/EBP α |
| Also known as | CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat;Monkey |
| Observed band | 42,also have 30kd isform |
| Cellular localization | Nucleus .; Isoform 4: Nucleus, nucleolus . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Non-alcoholic fatty liver disease;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Acute myeloid leukemia |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Thr226. AA range:192-241 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
4 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.