CD209 rabbit pAb
CD209 rabbit pAb from ELK Biotechnology Read more ›
Product Description
CD209 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CD209.
Background
This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino a
Additional antibody information
| Specificity | CD209 Polyclonal Antibody detects endogenous levels of CD209 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of mouse brain cells using CD209 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | CD209 |
| Also known as | CD209; CLEC4L; CD209 antigen; C-type lectin domain family 4 member L; Dendritic cell-specific ICAM-3-grabbing non-integrin 1; DC-SIGN; DC-SIGN1; CD209 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 45kD |
| Cellular localization | Isoform 1: Cell membrane ; Single-pass type II membrane protein .; Isoform 2: Cell membrane ; Single-pass type II membrane protein .; Isoform 3: Cell membrane ; Single-pass type II membrane protein .; Isoform 4: Cell membrane ; Single-pass type II membrane protein .; Isoform 5: Cell membrane ; Single-pass type II membrane protein .; Isoform 6: Secreted .; Isoform 7: Secreted .; Isoform 8: Secreted .; Isoform 9: Secreted .; Isoform 10: Secreted .; Isoform 11: Secreted .; Isoform 12: Secreted . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Phagosome;>>C-type lectin receptor signaling pathway;>>Tuberculosis;>>Measles |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | Synthesized peptide derived from CD209 antigen at AA range: 261-310 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.