Integrin β2 rabbit pAb from ELK Biotechnology Read more ›
Integrin β2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Integrin β2 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],
| Specificity | Integrin β2 Polyclonal Antibody detects endogenous levels of Integrin β2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HeLa cells using Integrin β2 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of KB-UV Hela using Integrin β2 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES4191 |
| Target | Integrin β2 |
| Research areas | >>Rap1 signaling pathway, >>Phagosome, >>Hippo signaling pathway, >>Cell adhesion molecules, >>Complement and coagulation cascades, >>Neutrophil extracellular trap formation, >>Natural killer cell mediated cytotoxicity, >>Leukocyte transendothelial migration, >>Regulation of actin cytoskeleton, >>Pertussis, >>Legionellosis, >>Leishmaniasis, >>Malaria, >>Amoebiasis, >>Staphylococcus aureus infection, >>Tuberculosis, >>Human T-cell leukemia virus 1 infection, >>Rheumatoid arthritis, >>Viral myocarditis |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human ITGB2. AA range:621-670 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Leukocytes (PubMed:23775590). Expressed in neutrophils (at protein level) (PubMed:21193407, PubMed:28807980). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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