SDHB rabbit pAb from ELK Biotechnology Read more ›
SDHB rabbit pAb is a research-use antibody from ELK Biotechnology for studying SDHB in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Fish. Specifications and supporting documents below should be reviewed when planning the experiment.
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008],
| Specificity | SDHB Polyclonal Antibody detects endogenous levels of SDHB protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HeLa, 293 cells using SDHB Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-muscle, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES4180 |
| Target | SDHB |
| Research areas | >>Citrate cycle (TCA cycle), >>Oxidative phosphorylation, >>Metabolic pathways, >>Carbon metabolism, >>Thermogenesis, >>Non-alcoholic fatty liver disease, >>Alzheimer disease, >>Parkinson disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Chemical carcinogenesis - reactive oxygen species, >>Diabetic cardiomyopathy |
| Reactivity | Human,Mouse,Rat,Fish |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human SDHB. AA range:131-180 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Fibroblast,Liver, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Fish |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Fish | — | — |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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