gp91-phox rabbit pAb from ELK Biotechnology Read more ›
gp91-phox rabbit pAb is a research-use antibody from ELK Biotechnology for studying gp91-phox in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008],
| Specificity | gp91-phox Polyclonal Antibody detects endogenous levels of gp91-phox protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of K562 cells using gp91-phox Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of HEPG2 using gp91-phox Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES4113 |
| Target | gp91-phox |
| Research areas | >>HIF-1 signaling pathway, >>Phagosome, >>Ferroptosis, >>Necroptosis, >>Neutrophil extracellular trap formation, >>NOD-like receptor signaling pathway, >>Leukocyte transendothelial migration, >>AGE-RAGE signaling pathway in diabetic complications, >>Alzheimer disease, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Leishmaniasis, >>Coronavirus disease - COVID-19, >>Diabetic cardiomyopathy, >>Lipid and atherosclerosis |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human CYBB. AA range:111-160 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Detected in neutrophils (at protein level). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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