Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98009

IGFBP6 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

IGFBP6 rabbit pAb from ELK Biotechnology Read more ›

IGFBP6 rabbit pAb
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IGFBP6 rabbit pAb

Product Description

IGFBP6 rabbit pAb is a research-use antibody from ELK Biotechnology for studying IGFBP6 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.,PTM:O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. Major glycoforms consist of 8-16 monosaccharides (by homology to IGFBP6 expressed recombinantly in CHO cells).,similarity:Contains 1 IGFBP N-terminal domain.,similarity:Contains 1 thyroglobulin type-1 domain.,

Additional antibody information

Specificity IGFBP6 Polyclonal Antibody detects endogenous levels of IGFBP6 protein.
Validation evidence captions Western Blot analysis of K562 cells using IGFBP6 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from K562 cells, using IGFBP6 Antibody.

Key Facts

Product typePrimary Antibody Target: IGFBP6
Collaborator ELK Biotechnology
Partner model ES4091
Target IGFBP6
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from the Internal region of human IGFBP6. AA range:101-150
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Cerebrospinal fluid,Fibroblast,Osteosarcoma,Pancreas,Placen
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
IGFBP6
Also known as
IGFBP6; IBP6; Insulin-like growth factor-binding protein 6; IBP-6; IGF-binding protein 6; IGFBP-6
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human IGFBP6. AA range:101-150
Observed band
25kD
Cellular localization
Secreted .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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