Manufactured by:ELK Biotechnology
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MyoD rabbit pAb

MyoD rabbit pAb from ELK Biotechnology

SKU:
ABM107857
Manufacturer SKU
ES4066
Product type
Primary Antibodies
TargetMyoD
ApplicationsWB, IHC
Species / reactivityHuman, Mouse, Rat, Chicken
Expression systemMuscle,Skeletal muscle,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

MyoD rabbit pAb is a research-use antibody from ELK Biotechnology for studying MyoD in WB, IHC workflows with listed reactivity for Human, Mouse, Rat, Chicken(testedbyourcustomer). Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity MyoD Polyclonal Antibody detects endogenous levels of MyoD protein.
Validation evidence captions Western Blot analysis of Jurkat cells using MyoD Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Western blot analysis of extracts from Jurkat cells, using MyoD (Ab-99/102) Antibody. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Key facts & specifications
Research areas
Spinocerebellar ataxia
Also known as
MYOD1; BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Immunogen
The antiserum was produced against synthesized peptide derived from human MyoD around the non-acetylation site of Lys99/102. AA range:61-110
Observed band
35kD
Cellular localization
Nucleus.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 61-110
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Chicken(testedbyourcustomer)
Identifiers & database references
Manufacturer SKU
ES4066
UniProt entry
P15172
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

MYOD1myogenic differentiation 1

View target guide

MYOD1 identifies myogenic differentiation 1 in Homo sapiens (Human). This target record connects NCBI Gene 4654, UniProtKB P15172 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
4654
UniProtKB
P15172
ELK Biotechnology
£220.00£176.00