Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97921

CD32-A rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CD32-A rabbit pAb from ELK Biotechnology Read more ›

CD32-A rabbit pAb
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CD32-A rabbit pAb

Product Description

CD32-A rabbit pAb is a research-use antibody from ELK Biotechnology for studying CD32-A in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Fc fragment of IgG receptor IIa(FCGR2A) Homo sapiens This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008],

Additional antibody information

Specificity CD32-A Polyclonal Antibody detects endogenous levels of CD32-A protein.
Validation evidence captions Western Blot analysis of K562 cells using CD32-A Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from K562 cells, using FCGR2A Antibody.

Key Facts

Product typePrimary Antibody Target: CD32-A
Collaborator ELK Biotechnology
Partner model ES3975
Target CD32-A
Research areas>>Phagosome, >>Osteoclast differentiation, >>Platelet activation, >>Neutrophil extracellular trap formation, >>Fc gamma R-mediated phagocytosis, >>Pathogenic Escherichia coli infection, >>Yersinia infection, >>Leishmaniasis, >>Staphylococcus aureus infection, >>Tuberculosis, >>Coronavirus disease - COVID-19, >>Systemic lupus erythematosus
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from the N-ternal region of human FCGR2A. AA range:41-90
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Found on monocytes, neutrophils and eosinophil platelets.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CD32-A
Also known as
FCGR2A; CD32; FCG2; FCGR2A1; IGFR2; Low affinity immunoglobulin gamma Fc region receptor II-a; IgG Fc receptor II-a; CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD32
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the N-ternal region of human FCGR2A. AA range:41-90
Observed band
34kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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