Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97905

Layilin rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Layilin rabbit pAb from ELK Biotechnology Read more ›

Layilin rabbit pAb
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Layilin rabbit pAb

Product Description

Layilin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Layilin in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

domain:The C-terminal domain interacts with the N-terminal domain of RDX.,function:Receptor for hyaluronate.,similarity:Contains 1 C-type lectin domain.,subcellular location:Colocalizes with TLN1 at the membrane ruffles.,subunit:Interacts with NF2, RDX and TLN1.,

Additional antibody information

Specificity Layilin Polyclonal Antibody detects endogenous levels of Layilin protein.
Validation evidence captions Western Blot analysis of HuvEc, K562 cells using Layilin Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100

Key Facts

Product typePrimary Antibody Target: Layilin
Collaborator ELK Biotechnology
Partner model ES3952
Target Layilin
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from the Internal region of human LAYN. AA range:71-120
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Layilin
Also known as
LAYN; Layilin
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human LAYN. AA range:71-120
Observed band
43kD
Cellular localization
Membrane ; Single-pass type I membrane protein . Colocalizes with TLN1 at the membrane ruffles. .
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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