TYR rabbit pAb from ELK Biotechnology Read more ›
TYR rabbit pAb is a research-use antibody from ELK Biotechnology for studying Tyrosinase in WB, ELISA workflows with listed reactivity for Human, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
tyrosinase(TYR) Homo sapiens The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008],
| Specificity | TYR Polyclonal Antibody detects endogenous levels of TYR protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using TYR Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of COS7 cells using TYR Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of various cell Lysate, antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysates from COS7 cells, treated with UV 30', using Tyrosinase Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3659 |
| Target | Tyrosinase |
| Research areas | >>Tyrosine metabolism, >>Metabolic pathways, >>Melanogenesis |
| Reactivity | Human,Monkey |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Tyrosinase. AA range:471-520 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Liver,Melanoma,Skin,T-cell, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Monkey | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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