ABM97743
TYR rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
TYR rabbit pAb from ELK Biotechnology Read more ›
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Product Description
TYR rabbit pAb is an ELK Biotechnology antibody for research workflows involving Tyrosinase.
Background
tyrosinase(TYR) Homo sapiens The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008],
Additional antibody information
| Specificity | TYR Polyclonal Antibody detects endogenous levels of TYR protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using TYR Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of COS7 cells using TYR Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of various cell Lysate, antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysates from COS7 cells, treated with UV 30', using Tyrosinase Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: Tyrosinase
| Target | Tyrosinase |
| Also known as | TYR; Tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Monkey |
| Observed band | 80kD |
| Cellular localization | Melanosome membrane ; Single-pass type I membrane protein . Melanosome . Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Tyrosine metabolism;>>Metabolic pathways;>>Melanogenesis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Tyrosinase. AA range:471-520 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Monkey |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Monkey | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Tyrosinase
Also known as
TYR; Tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Monkey
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human Tyrosinase. AA range:471-520
Observed band
80kD
Cellular localization
Melanosome membrane ; Single-pass type I membrane protein . Melanosome . Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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