TNF-β rabbit pAb from ELK Biotechnology Read more ›
TNF-β rabbit pAb is a research-use antibody from ELK Biotechnology for studying TNFB in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012],
| Specificity | TNF-β Polyclonal Antibody detects endogenous levels of TNF-β protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using TNF-β Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from COS-7cell, using TNF-β antibodys. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3624 |
| Target | TNFB |
| Research areas | >>Cytokine-cytokine receptor interaction, >>Viral protein interaction with cytokine and cytokine receptor, >>NF-kappa B signaling pathway, >>TNF signaling pathway, >>Type I diabetes mellitus, >>Human T-cell leukemia virus 1 infection, >>Herpes simplex virus 1 infection |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TNF-beta. AA range:17-66 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Lymph,Peripheral blood leukocyte,Peripheral blood mononuclear cells,Peripheral blood mononu |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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