SH-PTP2 rabbit pAb from ELK Biotechnology Read more ›
SH-PTP2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SH-PTP2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016],
| Specificity | SH-PTP2 Polyclonal Antibody detects endogenous levels of SH-PTP2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using SH-PTP2 Polyclonal Antibody diluted at 1:1000 | Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SHP-2 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from A431 cells, using SHP-2 Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3440 |
| Target | SH-PTP2 |
| Research areas | >>Ras signaling pathway, >>Phospholipase D signaling pathway, >>Axon guidance, >>C-type lectin receptor signaling pathway, >>JAK-STAT signaling pathway, >>Natural killer cell mediated cytotoxicity, >>Leukocyte transendothelial migration, >>Neurotrophin signaling pathway, >>Adipocytokine signaling pathway, >>Insulin resistance, >>Epithelial cell signaling in Helicobacter pylori infection, >>Pathogenic Escherichia coli infection, >>Herpes simplex virus 1 infection, >>Proteoglycans in cancer, >>Chemical carcinogenesis - reactive oxygen species, >>Renal cell carcinoma, >>Chronic myeloid leukemia, >>PD-L1 expression and PD-1 checkpoint pathway in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SHP-2. AA range:508-557 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed, with highest levels in heart, brain, and skeletal muscle. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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