Primary Antibody ISO 9001|ABMIUM Verified™
ABM97612

RFPL4A rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

RFPL4A rabbit pAb from ELK Biotechnology Read more ›

Related formats
RFPL4A rabbit pAb
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RFPL4A rabbit pAb

Product Description

RFPL4A rabbit pAb is an ELK Biotechnology antibody for research workflows involving RFPL4A.

Background

The ret finger protein-like 4 gene, Rfpl4, encodes a putative E3 ubiquitin-protein ligase expressed in adult germ cells.

Additional antibody information

Specificity RFPL4A Polyclonal Antibody detects endogenous levels of RFPL4A protein.
Validation evidence captions Western Blot analysis of various cells using RFPL4A Polyclonal Antibody | Western blot analysis of lysates from HUVEC cells, using RFPL4A Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using RFPL4A antibody. | Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product type Primary Antibody Target: RFPL4A
Target RFPL4A
Also known as RFPL4A; RFPL4; RNF210; Ret finger protein-like 4A; RING finger protein 210
Concentration 1 mg/ml
Working concentration WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Species reactivity Human;Rat;Mouse;
Observed band 42kD
Cellular localization Cytoplasm . Nucleus .
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area Antibody Research
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIHCELISA
Dilution range WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen The antiserum was produced against synthesized peptide derived from human RFPL4A. AA range:155-204
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
RFPL4A
Also known as
RFPL4A; RFPL4; RNF210; Ret finger protein-like 4A; RING finger protein 210
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human RFPL4A. AA range:155-204
Observed band
42kD
Cellular localization
Cytoplasm . Nucleus .
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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