Rabaptin-5β rabbit pAb from ELK Biotechnology Read more ›
Rabaptin-5β rabbit pAb is a research-use antibody from ELK Biotechnology for studying Rabaptin-5β in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
function:Plays a role in membrane trafficking and in homotypic early endosome fusion.,similarity:Belongs to the rabaptin family.,subunit:Heterodimer with RABGEF1. The dimer binds RAB5A that has been activated by GTP-binding.,
| Specificity | Rabaptin-5β Polyclonal Antibody detects endogenous levels of Rabaptin-5β protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Rabaptin-5β Polyclonal Antibody | Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Western blot analysis of lysates from HeLa, Jurkat, 293, and HUVEC cells, using RABEP2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using RABEP2 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3296 |
| Target | Rabaptin-5β |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RABEP2. AA range:353-402 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Hepatoma,Mammary gland,Testis, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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