NGFR p75 rabbit pAb from ELK Biotechnology Read more ›
NGFR p75 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NGFR p75 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008],
| Specificity | NGFR p75 Polyclonal Antibody detects endogenous levels of NGFR p75 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using NGFR p75 Polyclonal Antibody | Western Blot analysis of HepG2 cells using NGFR p75 Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using TNR16 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HepG2 cells, using TNR16 Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2960 |
| Target | NGFR p75 |
| Research areas | >>MAPK signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>Cytokine-cytokine receptor interaction, >>PI3K-Akt signaling pathway, >>Apoptosis - multiple species, >>Neurotrophin signaling pathway, >>Transcriptional misregulation in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TNR16. AA range:121-170 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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