Primary Antibody ISO 9001|ABMIUM Verified™
ABM97456

NGFR p75 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

NGFR p75 rabbit pAb from ELK Biotechnology Read more ›

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NGFR p75 rabbit pAb
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NGFR p75 rabbit pAb

Product Description

NGFR p75 rabbit pAb is an ELK Biotechnology antibody for research workflows involving NGFR p75.

Background

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity NGFR p75 Polyclonal Antibody detects endogenous levels of NGFR p75 protein.
Validation evidence captions Western Blot analysis of various cells using NGFR p75 Polyclonal Antibody | Western Blot analysis of HepG2 cells using NGFR p75 Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using TNR16 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HepG2 cells, using TNR16 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product type Primary Antibody Target: NGFR p75
Target NGFR p75
Also known as NGFR; TNFRSF16; Tumor necrosis factor receptor superfamily member 16; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; NGF receptor; p75 ICD; CD antigen CD271
Concentration 1 mg/ml
Working concentration Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Species reactivity Human;Mouse;Rat
Observed band 75kD
Cellular localization Cell membrane ; Single-pass type I membrane protein . Perikaryon . Cell projection, growth cone . Cell projection, dendritic spine .
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area >>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Cytokine-cytokine receptor interaction;>>PI3K-Akt signaling pathway;>>Apoptosis - multiple species;>>Neurotrophin signaling pathway;>>Transcriptional misregulation in cancer
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIFELISA
Dilution range Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen The antiserum was produced against synthesized peptide derived from human TNR16. AA range:121-170
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
NGFR p75
Also known as
NGFR; TNFRSF16; Tumor necrosis factor receptor superfamily member 16; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; NGF receptor; p75 ICD; CD antigen CD271
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human TNR16. AA range:121-170
Observed band
75kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Perikaryon . Cell projection, growth cone . Cell projection, dendritic spine .
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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