MEK-4 rabbit pAb from ELK Biotechnology Read more ›
MEK-4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MEK-4 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
| Specificity | MEK-4 Polyclonal Antibody detects endogenous levels of MEK-4 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using MEK-4 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from NIH/3T3 cells, using SEK1/MKK4 Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES2774 |
| Target | MEK-4 |
| Research areas | >>MAPK signaling pathway, >>ErbB signaling pathway, >>Toll-like receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>TNF signaling pathway, >>GnRH signaling pathway, >>Relaxin signaling pathway, >>Growth hormone synthesis, secretion and action, >>Alcoholic liver disease, >>Epithelial cell signaling in Helicobacter pylori infection, >>Salmonella infection, >>Yersinia infection, >>Chagas disease, >>Hepatitis B, >>Human T-cell leukemia virus 1 infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Epstein-Barr virus infection, >>Chemical carcinogenesis - reactive oxygen species, >>Lipid and atherosclerosis, >>Fluid shear stress and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SEK1/MKK4. AA range:46-95 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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