IRAK-1 rabbit pAb from ELK Biotechnology Read more ›
IRAK-1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying IRAK-1 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
| Specificity | IRAK-1 Polyclonal Antibody detects endogenous levels of IRAK-1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using IRAK-1 Polyclonal Antibody | Western blot analysis of lysates from HeLa cells, using IRAK1 Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES2641 |
| Target | IRAK-1 |
| Research areas | >>MAPK signaling pathway, >>NF-kappa B signaling pathway, >>Toll-like receptor signaling pathway, >>Neurotrophin signaling pathway, >>Alcoholic liver disease, >>Pathogenic Escherichia coli infection, >>Salmonella infection, >>Pertussis, >>Yersinia infection, >>Leishmaniasis, >>Chagas disease, >>Toxoplasmosis, >>Tuberculosis, >>Hepatitis B, >>Measles, >>Herpes simplex virus 1 infection, >>Epstein-Barr virus infection, >>Human immunodeficiency virus 1 infection, >>Coronavirus disease - COVID-19, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IRAK1. AA range:353-402 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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