HSC 70 rabbit pAb from ELK Biotechnology Read more ›
HSC 70 rabbit pAb is a research-use antibody from ELK Biotechnology for studying HSC 70 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011],
| Specificity | HSC 70 Polyclonal Antibody detects endogenous levels of HSC 70 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using HSC 70 Polyclonal Antibody diluted at 1:2000 | Western Blot analysis of HepG2 cells using HSC 70 Polyclonal Antibody diluted at 1:2000 | Western blot analysis of lysate from HeLa cells, using HSC 70 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2569 |
| Target | HSC 70 |
| Research areas | >>Spliceosome, >>MAPK signaling pathway, >>Protein processing in endoplasmic reticulum, >>Endocytosis, >>Longevity regulating pathway - multiple species, >>Antigen processing and presentation, >>Estrogen signaling pathway, >>Prion disease, >>Legionellosis, >>Toxoplasmosis, >>Measles, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSC 70. AA range:202-251 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitous. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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