GCK rabbit pAb
GCK rabbit pAb from ELK Biotechnology Read more ›
Product Description
GCK rabbit pAb is an ELK Biotechnology antibody for research workflows involving GCK.
Background
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009],
Additional antibody information
| Specificity | GCK Polyclonal Antibody detects endogenous levels of GCK protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using GCK Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of NIH-3T3 cells using GCK Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysate from NIH/3T3 cells, using GCK antibody. |
Key Facts
| Target | GCK |
| Also known as | GCK; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 55kD |
| Cellular localization | Cytoplasm . Nucleus . Mitochondrion . Under low glucose concentrations, GCK associates with GCKR and the inactive complex is recruited to the hepatocyte nucleus. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Glycolysis / Gluconeogenesis;>>Galactose metabolism;>>Starch and sucrose metabolism;>>Amino sugar and nucleotide sugar metabolism;>>Neomycin, kanamycin and gentamicin biosynthesis;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of nucleotide sugars;>>Insulin signaling pathway;>>Insulin secretion;>>Prolactin signaling pathway;>>Glucagon signaling pathway;>>Type II diabetes mellitus;>>Maturity onset diabetes of the young;>>Central carbon metabolism in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GCK. AA range:43-92 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.