GCK rabbit pAb from ELK Biotechnology Read more ›
GCK rabbit pAb is a research-use antibody from ELK Biotechnology for studying GCK in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009],
| Specificity | GCK Polyclonal Antibody detects endogenous levels of GCK protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using GCK Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of NIH-3T3 cells using GCK Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysate from NIH/3T3 cells, using GCK antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2408 |
| Target | GCK |
| Research areas | >>Glycolysis / Gluconeogenesis, >>Galactose metabolism, >>Starch and sucrose metabolism, >>Amino sugar and nucleotide sugar metabolism, >>Neomycin, kanamycin and gentamicin biosynthesis, >>Metabolic pathways, >>Carbon metabolism, >>Biosynthesis of nucleotide sugars, >>Insulin signaling pathway, >>Insulin secretion, >>Prolactin signaling pathway, >>Glucagon signaling pathway, >>Type II diabetes mellitus, >>Maturity onset diabetes of the young, >>Central carbon metabolism in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GCK. AA range:43-92 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Lung,Pancreas,Placenta, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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