Estrogen Receptor α rabbit pAb
Estrogen Receptor α rabbit pAb from ELK Biotechnology Read more ›
Product Description
Estrogen Receptor α rabbit pAb is an ELK Biotechnology antibody for research workflows involving Estrogen Receptor-α.
Background
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014],
Additional antibody information
| Specificity | The antibody detects endogenous Estrogen Receptor α protein |
|---|---|
| Validation evidence captions | Western blot analysis of 1) Hela, 2)MCF7, 3) Mouse Liver Tissue, 4) Rat Liver Tissue with Estrogen Receptor a Rabbit pAb diluted at 1:2,000. |
Key Facts
| Target | Estrogen Receptor-α |
| Also known as | Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1) |
| Concentration | 1 mg/ml |
| Working concentration | WB: 1:500-1:2000 |
| Species reactivity | Human;Rat;Mouse |
| Observed band | 66kD |
| Cellular localization | Isoform 1: Nucleus . Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . A minor fraction is associated with the inner membrane.; Isoform 3: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Single-pass type I membrane protein. Associated with the inner membrane via palmitoylation (Probable). At least a subset exists as a transmembrane protein with a N-terminal extracellular domain. .; Nucleus. Golgi apparatus. Cell membrane. Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Endocrine resistance;>>Estrogen signaling pathway;>>Prolactin signaling pathway;>>Thyroid hormone signaling pathway;>>Endocrine and other factor-regulated calcium reabsorption;>>Pathways in cancer;>>Proteoglycans in cancer;>>Chemical carcinogenesis - receptor activation;>>Breast cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:500-1:2000 |
| Immunogen | Recombinant Protein of Estrogen Receptor α |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-1:2000 | — |
| Rat | — | — |
| Mouse | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.