Estrogen Receptor α rabbit pAb from ELK Biotechnology Read more ›
Estrogen Receptor α rabbit pAb is a research-use antibody from ELK Biotechnology for studying Estrogen Receptor-α in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014],
| Specificity | The antibody detects endogenous Estrogen Receptor α protein |
|---|---|
| Validation evidence captions | Western blot analysis of 1) Hela, 2)MCF7, 3) Mouse Liver Tissue, 4) Rat Liver Tissue with Estrogen Receptor a Rabbit pAb diluted at 1:2,000. |
| Collaborator | ELK Biotechnology |
| Partner model | ES20757 |
| Target | Estrogen Receptor-α |
| Research areas | >>Endocrine resistance, >>Estrogen signaling pathway, >>Prolactin signaling pathway, >>Thyroid hormone signaling pathway, >>Endocrine and other factor-regulated calcium reabsorption, >>Pathways in cancer, >>Proteoglycans in cancer, >>Chemical carcinogenesis - receptor activation, >>Breast cancer |
| Reactivity | Human,Rat,Mouse |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB: 1:500-1:2000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Protein of Estrogen Receptor α |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed (PubMed:10970861). Not expressed in the pituitary gland (PubMed:10970861). ; Isoform 3: Widely expressed, however not expressed in the pituitary gland. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-1:2000 | — |
| Rat | — | — |
| Mouse | — | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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