Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96644

Beta-defensin 4A Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Beta-defensin 4A Rabbit pAb from ELK Biotechnology Read more ›

Beta-defensin 4A Rabbit pAb
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Beta-defensin 4A Rabbit pAb

Product Description

Beta-defensin 4A Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Beta-defensin 4A in IHC, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of human Beta-defensin 4A
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Key Facts

Product typePrimary Antibody Target: Beta-defensin 4A
Collaborator ELK Biotechnology
Partner model ES18063
Target Beta-defensin 4A
Research areas>>NOD-like receptor signaling pathway, >>IL-17 signaling pathway, >>Staphylococcus aureus infection
Reactivity Human,Rat,Mouse
Applications IHC,ELISA
ConjugationUnconjugated
Dilution rangeIHC-p 1:50-200, ELISA(peptide)1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human Beta-defensin 4A AA range: 1-50
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in the skin and respiratory tract.
Purity The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC-p 1:50-200
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Beta-defensin 4A
Also known as
Beta-defensin 4A (Beta-defensin 2;BD-2;hBD-2;Defensin, beta 2;Skin-antimicrobial peptide 1;SAP1)
Concentration
1 mg/ml
Working concentration
IHC-p 1:50-200, ELISA(peptide)1:5000-20000
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
IHCELISA
Immunogen
Synthesized peptide derived from human Beta-defensin 4A AA range: 1-50
Cellular localization
Secreted.
Dilution range
IHC-p 1:50-200, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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