c-Abl (phospho-Tyr89) rabbit pAb
c-Abl (phospho-Tyr89) rabbit pAb from ELK Biotechnology Read more ›
Product Description
c-Abl (phospho-Tyr89) rabbit pAb is an ELK Biotechnology antibody for research workflows involving ABL1.
Background
This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [pr
Additional antibody information
| Specificity | This antibody detects endogenous levels of Human c-Abl (phospho-Tyr89) |
|---|---|
| Validation evidence captions | Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
Key Facts
| Target | ABL1 |
| Also known as | Tyrosine-protein kinase ABL1 (EC 2.7.10.2) (Abelson murine leukemia viral oncogene homolog 1) (Abelson tyrosine-protein kinase 1) (Proto-oncogene c-Abl) (p150) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:1000-2000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 140(200kd BCR-ABL complex） |
| Cellular localization | Cytoplasm, cytoskeleton. Nucleus. Mitochondrion . Shuttles between the nucleus and cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress (By similarity). .; Isoform IB: Nucleus membrane; Lipid-anchor. The myristoylated c-ABL protein is reported to be nuclear. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>ErbB signaling pathway;>>Ras signaling pathway;>>Cell cycle;>>Axon guidance;>>Neurotrophin signaling pathway;>>Pathogenic Escherichia coli infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Chronic myeloid leukemia;>>Viral myocarditis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:1000-2000 |
| Immunogen | Synthesized phosho peptide around human c-Abl (Tyr89) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 | — |
| Rat | — | — |
| Mouse | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.