CCL18 Rabbit pAb
CCL18 Rabbit pAb from ELK Biotechnology Read more ›
Product Description
CCL18 Rabbit pAb is an ELK Biotechnology antibody for research workflows involving CCL18.
Background
This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for naive T cells, CD4+ and CD8+ T cells and nonactivated lymphocytes, but not for monocytes or granulocytes. This chemokine attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes. It may play a role in both humoral and cell-mediated immunity responses. [provided by RefSeq, Sep 2014],
Additional antibody information
| Specificity | This antibody detects endogenous levels of human CCL18 |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
Key Facts
| Target | CCL18 |
| Also known as | C-C motif chemokine 18 (Alternative macrophage activation-associated CC chemokine 1;AMAC-1;CC chemokine PARC;Dendritic cell chemokine 1;DC-CK1;Macrophage inflammatory protein 4;MIP-4;Pulmonary and activation-regulated chemokine;Small-inducible cytokine A18) Cleaved into: CCL18(1-68); CCL18(3-69); CCL18(4-69) |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p 1:50-200, ELISA(peptide)1:5000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Cellular localization | Secreted. |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cytokine-cytokine receptor interaction;>>Viral protein interaction with cytokine and cytokine receptor;>>Chemokine signaling pathway |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCELISA |
| Dilution range | IHC-p 1:50-200, ELISA(peptide)1:5000-20000 |
| Immunogen | Synthesized peptide derived from human CCL18 AA range: 1-80 |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:50-200 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.