Arginase II rabbit pAb
Arginase II rabbit pAb from ELK Biotechnology Read more ›
Product Description
Arginase II rabbit pAb is an ELK Biotechnology antibody for research workflows involving Arginase II.
Background
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Arginase II Polyclonal Antibody detects endogenous levels of Arginase II protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Arginase II Polyclonal Antibody | Western blot analysis of the lysates from HeLa cells using ARG2 antibody. |
Key Facts
| Target | Arginase II |
| Also known as | ARG2; Arginase-2; mitochondrial; Kidney-type arginase; Non-hepatic arginase; Type II arginase |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 38kD |
| Cellular localization | Mitochondrion . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Arginine biosynthesis;>>Arginine and proline metabolism;>>Metabolic pathways;>>Biosynthesis of amino acids;>>Amoebiasis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ARG2. AA range:305-354 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.