DHSO rabbit pAb from ELK Biotechnology Read more ›
DHSO rabbit pAb is a research-use antibody from ELK Biotechnology for studying DHSO in WB, IP workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010],
| Specificity | This antibody detects endogenous levels of DHSO at Human/Mouse/Rat |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night | 1) Input: Hela Lysate 2) IP product: IP dilute 1: 200 Western blot analysis: primary antibody : 1:2000 Secondary antibody: Goat anti-Mouse IgG(RS0002), 1: 5000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES16949 |
| Target | DHSO |
| Research areas | >>Pentose and glucuronate interconversions, >>Fructose and mannose metabolism, >>Metabolic pathways |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IP |
| Conjugation | Unconjugated |
| Dilution range | WB 1：500-2000 IP 1:200 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human DHSO |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in liver (PubMed:3365415). Expressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level). |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1：500-2000 IP 1:200 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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