Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95884

Flotillin-1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Flotillin-1 rabbit pAb from ELK Biotechnology Read more ›

Flotillin-1 rabbit pAb
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Flotillin-1 rabbit pAb

Product Description

Flotillin-1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Flotillin-1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016],

Additional antibody information

Specificity This antibody detects endogenous levels of human Flotillin-1
Validation evidence captions Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour.

Key Facts

Product typePrimary Antibody Target: Flotillin-1
Collaborator ELK Biotechnology
Partner model ES16367
Target Flotillin-1
Research areas>>Insulin signaling pathway
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA(peptide)1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human Flotillin-1
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Adipose tissue,Adrenal gland,Lung,Peripheral blood leukocyte,Umbilical cord blood,
Purity The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Flotillin-1
Also known as
Flotillin-1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human Flotillin-1
Observed band
48kD
Cellular localization
Cell membrane; Peripheral membrane protein . Endosome . Membrane, caveola ; Peripheral membrane protein . Melanosome . Membrane raft . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Membrane-associated protein of caveola (By similarity). .
Dilution range
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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