Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95564

JunB (phospho-Thr102/Thr104) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

JunB (phospho-Thr102/Thr104) rabbit pAb from ELK Biotechnology Read more ›

JunB (phospho-Thr102/Thr104) rabbit pAb
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JunB (phospho-Thr102/Thr104) rabbit pAb

Product Description

JunB (phospho-Thr102/Thr104) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Jun B in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of Human JunB (phospho-Thr102 or Thr104)
Validation evidence captions Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: Jun B
Collaborator ELK Biotechnology
Partner model ES15381
Target Jun B
Research areas>>Osteoclast differentiation, >>TNF signaling pathway, >>Growth hormone synthesis, secretion and action
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phosho peptide around human JunB (Thr102 and Thr104)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Adipose tissue,Epithelium,Pancreas,Placenta,Prostat
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Jun B
Also known as
Transcription factor jun-B
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Synthesized phosho peptide around human JunB (Thr102 and Thr104)
Observed band
36kD
Cellular localization
Nucleus.
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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