MLKL (phospho Ser358) rabbit pAb from ELK Biotechnology Read more ›
MLKL (phospho Ser358) rabbit pAb is a research-use antibody from ELK Biotechnology for studying MLKL in WB, IHC, IF workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015],
| Specificity | This antibody detects endogenous phospho levels of MLKL (phospho S358) at Human, Mouse, Rat |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200 |
| Collaborator | ELK Biotechnology |
| Partner model | ES14935 |
| Target | MLKL |
| Research areas | >>Necroptosis, >>TNF signaling pathway, >>Salmonella infection |
| Reactivity | Human |
| Applications | WB,IHC,IF |
| Conjugation | Unconjugated |
| Dilution range | IHC-p 1:100-500;IF/ICC 1:100-500;WB 1:500-2000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human MLKL (phospho S358) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Chondrocyte,Leukocyte,Lymph node, |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:100-500;IF/ICC 1:100-500;WB 1:500-2000 | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
