MLKL (phospho Ser358) rabbit pAb
MLKL (phospho Ser358) rabbit pAb from ELK Biotechnology Read more ›
Product Description
MLKL (phospho Ser358) rabbit pAb is an ELK Biotechnology antibody for research workflows involving MLKL.
Background
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015],
Additional antibody information
| Specificity | This antibody detects endogenous phospho levels of MLKL (phospho S358) at Human, Mouse, Rat |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200 |
Key Facts
| Target | MLKL |
| Also known as | Mixed lineage kinase domain-like protein |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p 1:100-500;IF/ICC 1:100-500;WB 1:500-2000 |
| Species reactivity | Human |
| Observed band | 55kD |
| Cellular localization | Cytoplasm . Cell membrane . Nucleus . Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (PubMed:24316671). Localizes to the nucleus in response to orthomyxoviruses infection (By similarity). . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Necroptosis;>>TNF signaling pathway;>>Salmonella infection |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | IHC-p 1:100-500;IF/ICC 1:100-500;WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human MLKL (phospho S358) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:100-500;IF/ICC 1:100-500;WB 1:500-2000 | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.