PPAR-γ (phospho Ser112) rabbit pAb from ELK Biotechnology Read more ›
PPAR-γ (phospho Ser112) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PPAR-γ in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
peroxisome proliferator activated receptor gamma(PPARG) Homo sapiens This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-PPAR-γ (S112) Polyclonal Antibody detects endogenous levels of PPAR-γ protein only when phosphorylated at S112. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-PPAR-γ (S112) Polyclonal Antibody diluted at 1:500 | Western Blot analysis of PC-3 cells using Phospho-PPAR-γ (S112) Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from Jurkat cells treated with Paclitaxel 1uM 24h, using PPAR-gamma (Phospho-Ser112) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1428 |
| Target | PPAR-γ |
| Research areas | >>PPAR signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Osteoclast differentiation, >>Thermogenesis, >>Non-alcoholic fatty liver disease, >>Huntington disease, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Thyroid cancer, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PPAR-gamma around the phosphorylation site of Ser112. AA range:78-127 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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