PPAR-γ (phospho Ser112) rabbit pAb
PPAR-γ (phospho Ser112) rabbit pAb from ELK Biotechnology Read more ›
Product Description
PPAR-γ (phospho Ser112) rabbit pAb is an ELK Biotechnology antibody for research workflows involving PPAR-γ.
Background
peroxisome proliferator activated receptor gamma(PPARG) Homo sapiens This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Phospho-PPAR-γ (S112) Polyclonal Antibody detects endogenous levels of PPAR-γ protein only when phosphorylated at S112. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-PPAR-γ (S112) Polyclonal Antibody diluted at 1:500 | Western Blot analysis of PC-3 cells using Phospho-PPAR-γ (S112) Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from Jurkat cells treated with Paclitaxel 1uM 24h, using PPAR-gamma (Phospho-Ser112) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
| Target | PPAR-γ |
| Also known as | PPARG; NR1C3; Peroxisome proliferator-activated receptor gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 60kD |
| Cellular localization | Nucleus. Cytoplasm. Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner. NOCT enhances its nuclear translocation. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>PPAR signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Osteoclast differentiation;>>Thermogenesis;>>Non-alcoholic fatty liver disease;>>Huntington disease;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Thyroid cancer;>>Lipid and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PPAR-gamma around the phosphorylation site of Ser112. AA range:78-127 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.