PDLI2 rabbit pAb
PDLI2 rabbit pAb from ELK Biotechnology Read more ›
Product Description
PDLI2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving PDLI2.
Background
This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein, and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011],
Additional antibody information
| Specificity | This antibody detects endogenous levels of PDLI2 at Human/Mouse/Rat |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | PDLI2 |
| Also known as | PDZ and LIM domain protein 2 (PDZ-LIM protein mystique) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 |
| Species reactivity | Human; Mouse;Rat |
| Observed band | 38kD |
| Cellular localization | Cytoplasm . Nucleus . May be partially nuclear.; Isoform 1: Cytoplasm, cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin (PXN).; Isoform 2: Cytoplasm, cytoskeleton.; Isoform 3: Nucleus. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.22% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human PDLI2 AA range: 190-240 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.