Stat6 (phospho Tyr641) rabbit pAb
Stat6 (phospho Tyr641) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Stat6 (phospho Tyr641) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Stat6.
Background
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],
Additional antibody information
| Specificity | Phospho-Stat6 (Y641) Polyclonal Antibody detects endogenous levels of Stat6 protein only when phosphorylated at Y641. |
|---|---|
| Validation evidence captions | Western blot analysis of Stat6 (phospho Tyr641) Polyclonal Antibody, using Hela,Jurkat cell treated or untreated with IFN 2500U/ml 30', 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour. | Western Blot analysis of various cells using Phospho-Stat6 (Y641) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using STAT6 (Phospho-Tyr641) Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT6 (Phospho-Tyr641) Antibody. The picture on the right is blocked with the phospho peptide. |
Key Facts
| Target | Stat6 |
| Also known as | STAT6; Signal transducer and activator of transcription 6; IL-4 Stat |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 94kD |
| Cellular localization | Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Necroptosis;>>JAK-STAT signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>Hepatitis B;>>Pathways in cancer;>>Inflammatory bowel disease |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human STAT6 around the phosphorylation site of Tyr641. AA range:608-657 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.