Stat6 (phospho Thr645) rabbit pAb from ELK Biotechnology Read more ›
Stat6 (phospho Thr645) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Stat6 in WB, IHC, IF, ELISA, IP workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],
| Specificity | Phospho-Stat6 (T645) Polyclonal Antibody detects endogenous levels of Stat6 protein only when phosphorylated at T645. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Stat6 (T645) Polyclonal Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT6 (Phospho-Thr645) Antibody. The picture on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1408 |
| Target | Stat6 |
| Research areas | >>Necroptosis, >>JAK-STAT signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>Hepatitis B, >>Pathways in cancer, >>Inflammatory bowel disease |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA,IP |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human STAT6 around the phosphorylation site of Thr645. AA range:612-661 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Uterus, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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