Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94863

PPR42 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

PPR42 rabbit pAb from ELK Biotechnology Read more ›

PPR42 rabbit pAb
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PPR42 rabbit pAb

Product Description

PPR42 rabbit pAb is a research-use antibody from ELK Biotechnology for studying PPR42 in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of PPR42 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from HuvEc cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: PPR42
Collaborator ELK Biotechnology
Partner model ES13986
Target PPR42
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human PPR42 AA range: 4-54
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Testis,
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse

Specifications

Target
PPR42
Also known as
Protein phosphatase 1 regulatory subunit 42 (Leucine-rich repeat-containing protein 67)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human PPR42 AA range: 4-54
Observed band
38kD
Cellular localization
Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes with alpha tubulin to the manchette of developing spermatids. Detected to nuclear rim in pachytene spermatocytes. Detected at nuclear surface, opposite the acrosome in elongating spermatids. Detected at the microtubule-organizing center (MTOC). Localized to the centrosomal region of late-stage spermatids (By similarity). .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.71% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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