p27 (phospho Ser10) rabbit pAb from ELK Biotechnology Read more ›
p27 (phospho Ser10) rabbit pAb is a research-use antibody from ELK Biotechnology for studying p27 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014],
| Specificity | Phospho-p27 (S10) Polyclonal Antibody detects endogenous levels of p27 protein only when phosphorylated at S10. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-p27 (S10) Polyclonal Antibody | Western blot analysis of lysates from A2780 and COLO205 cells, using p27 Kip1 (Phospho-Ser10) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1378 |
| Target | p27 |
| Research areas | >>Endocrine resistance, >>ErbB signaling pathway, >>HIF-1 signaling pathway, >>FoxO signaling pathway, >>Cell cycle, >>PI3K-Akt signaling pathway, >>AGE-RAGE signaling pathway in diabetic complications, >>Cushing syndrome, >>Measles, >>Human papillomavirus infection, >>Epstein-Barr virus infection, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Viral carcinogenesis, >>MicroRNAs in cancer, >>Prostate cancer, >>Chronic myeloid leukemia, >>Small cell lung cancer, >>Gastric cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p27 Kip1 around the phosphorylation site of Ser10. AA range:1-50 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in kidney (at protein level) (PubMed:15509543). Expressed in all tissues tested (PubMed:8033212). Highest levels in skeletal muscle, lowest in liver and kidney (PubMed:8033212). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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