ABM94753
JAK2 (phospho Tyr1007) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
JAK2 (phospho Tyr1007) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
JAK2 (phospho Tyr1007) rabbit pAb is an ELK Biotechnology antibody for research workflows involving JAK2.
Background
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Phospho-JAK2 (Y1007) Polyclonal Antibody detects endogenous levels of JAK2 protein only when phosphorylated at Y1007. |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
Key Facts
Product type
Primary Antibody›
Target: JAK2
| Target | JAK2 |
| Also known as | JAK2; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2 |
| Concentration | 1 mg/ml |
| Working concentration | Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 130kD |
| Cellular localization | Endomembrane system ; Peripheral membrane protein . Cytoplasm . Nucleus . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>EGFR tyrosine kinase inhibitor resistance;>>Chemokine signaling pathway;>>PI3K-Akt signaling pathway;>>Necroptosis;>>Signaling pathways regulating pluripotency of stem cells;>>JAK-STAT signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>Cholinergic synapse;>>Prolactin signaling pathway;>>Adipocytokine signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Growth hormone synthesis, secretion and action;>>Leishmaniasis;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis B;>>Influenza A;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Pathways in cancer;>>Chemical carcinogenesis - receptor activation;>>PD-L1 expression and PD-1 checkpoint pathway in cancer;>>Lipid and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr1007. AA range:981-1030 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
JAK2
Also known as
JAK2; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2
Concentration
1 mg/ml
Working concentration
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr1007. AA range:981-1030
Observed band
130kD
Cellular localization
Endomembrane system ; Peripheral membrane protein . Cytoplasm . Nucleus .
Dilution range
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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