JAK2 (phospho Tyr1007) rabbit pAb from ELK Biotechnology Read more ›
JAK2 (phospho Tyr1007) rabbit pAb is a research-use antibody from ELK Biotechnology for studying JAK2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-JAK2 (Y1007) Polyclonal Antibody detects endogenous levels of JAK2 protein only when phosphorylated at Y1007. |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1348 |
| Target | JAK2 |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Chemokine signaling pathway, >>PI3K-Akt signaling pathway, >>Necroptosis, >>Signaling pathways regulating pluripotency of stem cells, >>JAK-STAT signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>Cholinergic synapse, >>Prolactin signaling pathway, >>Adipocytokine signaling pathway, >>AGE-RAGE signaling pathway in diabetic complications, >>Growth hormone synthesis, secretion and action, >>Leishmaniasis, >>Toxoplasmosis, >>Tuberculosis, >>Hepatitis B, >>Influenza A, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 infection, >>Pathways in cancer, >>Chemical carcinogenesis - receptor activation, >>PD-L1 expression and PD-1 checkpoint pathway in cancer, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr1007. AA range:981-1030 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitously expressed throughout most tissues. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
