IκB-α (phospho Ser32/S36) rabbit pAb from ELK Biotechnology Read more ›
IκB-α (phospho Ser32/S36) rabbit pAb is a research-use antibody from ELK Biotechnology for studying IκB-α in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011],
| Specificity | Phospho-IκB-α (S32/S36) Polyclonal Antibody detects endogenous levels of IκB-α protein only when phosphorylated at S32/S36. |
|---|
| Collaborator | ELK Biotechnology |
| Partner model | ES1345 |
| Target | IκB-α |
| Research areas | >>cAMP signaling pathway, >>Chemokine signaling pathway, >>NF-kappa B signaling pathway, >>Apoptosis, >>Osteoclast differentiation, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>Cytosolic DNA-sensing pathway, >>C-type lectin receptor signaling pathway, >>IL-17 signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>TNF signaling pathway, >>Neurotrophin signaling pathway, >>Adipocytokine signaling pathway, >>Relaxin signaling pathway, >>Insulin resistance, >>Alcoholic liver disease, >>Epithelial cell signaling in Helicobacter pylori infection, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Salmonella infection, >>Legionellosis, >>Yersinia infection, >>Leishmaniasis, >>Chagas disease, >>Toxoplasmosis, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Human cytomegalovirus infection, >>Influenza A, >>Human T-cell leukemia virus 1 infection, >> |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Ser32/Ser36. AA range:15-64 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Kidney,Lymph node,Monocyte, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
