HDAC4 (phospho Ser632) rabbit pAb from ELK Biotechnology Read more ›
HDAC4 (phospho Ser632) rabbit pAb is a research-use antibody from ELK Biotechnology for studying HDAC4 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-HDAC4 (S632) Polyclonal Antibody detects endogenous levels of HDAC4 protein only when phosphorylated at S632. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-HDAC4 (S632) Polyclonal Antibody diluted at 1:1000 | Western blot analysis of HELA SH-SY5Y 3T3 lysis using Phospho-HDAC4 (S632) antibody. Antibody was diluted at 1:1000 | Western blot analysis of lysates from 293 cells treated with etoposide 25uM 1hour and Jurkat cells treated with etoposide 25uM 24hours, using HDAC4 (Phospho-Ser632) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1323 |
| Target | HDAC4 |
| Research areas | >>Apelin signaling pathway, >>Neutrophil extracellular trap formation, >>Alcoholism, >>Viral carcinogenesis, >>MicroRNAs in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HDAC4 around the phosphorylation site of Ser632. AA range:598-647 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitous. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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